Regulatory
PREmR34

Part:BBa_K5097001:Design

Designed by: Kelly Acen, Marie Box, Alexia Darby, Jena Dookie, Blake Dieckman, Runya Chaora, Chris Dorce, Adrianna Dugan, Elber Lopez-Hernandez, kayla VanPelt, Makenna Ventuleth   Group: iGEM24_Oneonta   (2024-09-30)


pH Response Element riboswitch mR34


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 126


Design Notes

Putative rbs with weak affinity of PRE, was replaced with a strong rbs.


Source

Improved version of PRE (BBa_K2348000) loci from E.coli genome. Contains a strong ribosome binding site (BBa_B0034).