Regulatory
PREmR34
Part:BBa_K5097001:Design
Designed by: Kelly Acen, Marie Box, Alexia Darby, Jena Dookie, Blake Dieckman, Runya Chaora, Chris Dorce, Adrianna Dugan, Elber Lopez-Hernandez, kayla VanPelt, Makenna Ventuleth Group: iGEM24_Oneonta (2024-09-30)
pH Response Element riboswitch mR34
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 126
Design Notes
Putative rbs with weak affinity of PRE, was replaced with a strong rbs.
Source
Improved version of PRE (BBa_K2348000) loci from E.coli genome. Contains a strong ribosome binding site (BBa_B0034).